Orthotopic PDX testing: How it works.

Certis Oncology Solutions’ orthotopic PDX testing provides your doctors with the most accurate (and fastest) data about how different drugs work against your stage 2, 3 or 4 cancer. And more than 40 studies published in peer-reviewed scientific journals have validated that it works well. But how does it work?.


Striving for personalized cancer care.

How your tumor grows, changes, regresses and otherwise responds to a particular drug is powerful knowledge for cancer doctors. It allows doctors to avoid treatments that would be ineffective for your one-of-a-kind cancer…and avoid the unnecessary toxic exposure that comes with them.

With this knowledge, your doctor can leap straight to the drug therapy that will be most effective so you can have more time and a higher quality of life. It could even save your life.

The problem is that cancer doctors don’t have this information. Truly personalized cancer treatment is a current focus and goal of cancer care, but even the most modern protocols are based on much broader science.

The treatment that doctors recommend today is based on what works for different types of cancers, certain stages, specific cell types, and other factors. Cancer treatment is more advanced and effective than ever, but it still isn’t specific to your unique tumor.

How Certis’ orthotopic PDX testing is making treatment more individualized.

Certis’ orthotopic PDX testing is changing that. It works in a few key ways:

  • We don’t test the drugs on you – With the toxicity and risks of anti-cancer drugs, it’s not possible to try different treatments on you. Actually, because they are based on group findings and not personalized to you, standard-of-care treatments are essentially a “best-guess” attempt to see how well your cancer responds.
  • We test the various drugs on special immunodeficient mice – These aren’t your typical pet-store mice. The mice do not have the immune defenses with which to fight your tumor, which means that your tumor tissue will grow.
  • We test multiple drugs at the same time – Implanting your tumor tissue in multiple mice enables us to test several drugs concurrently, usually four or five of them. Think of this as four or five cancer treatment protocols being simultaneously administered, which could never happen.
  • We implant your tumor into the mice orthotopically – Rather than implant your tumor tissue into the mice subcutaneously (“under the skin”), we implant it orthotopically, meaning “in the correct place.” If you have a liver tumor, we surgically and precisely implant it as a PDX into the mice’s livers. Whether it’s a pancreas tumor, a breast tumor or any other kind, we implant it where it’s supposed to go.
  • Orthotopic PDXs offer the highest clinical relevance to your tumor – Peer-reviewed scientific studies show that orthotopic PDXs, challenging as they are to implant into mice, provide the highest degree of clinical correlation to the tumor inside your body. Basically, how the tumor responds in the mice is how it would respond in you.
  • Orthotopic PDXs provide for the most accurate results Orthotopic PDXs are less susceptible to false positives and negatives than subcutaneous PDXs. They also metastasize similarly to human tumors, allowing us to test drugs not just on primary tumors but also on metastatic tumors. In addition, orthotopic PDXs have been shown to maintain the genetic fidelity from your tissue sample to the mice and through several passages (generations) of the tumor.
  • Orthotopic xenografts offer the shortest time from implantation to results – The time it takes to get the scientific data your doctor needs is as important as the accuracy of that data. As one of our foremost goals is to help your doctor give you more time, speed matters. And because we can use tumor tissue from a core needle biopsy, we can get started as early as possible for even faster results.

For more information about Certis’ testing, how it works, or how to get started, call 858-228-4110.